We Provide

These services are designed to provide accurate and reliable results, enabling clients to make informed decisions and achieve their desired outcomes.

Anti-cancer activity

A. Tryphan blue dye exclusion method
B. Cell Viability Assay by MTT
C. Cell Viability Assay by SRB
D. Cell Viability Assay by XTT
E. Cell Viability Assay by WST
F. Cytotoxic Evolution In Neutrophils By Cell Morphology
G. Cell Viability Assay by Crysta

In vitro Apoptosis Determination

A. Apoptosis determination by AO/EB Staining Method
B. Apoptosis determination by DNA Fragmentation using Flow cytometry
C. Apoptosis determination by DAPI Staining
D. Apoptosis determination in Mitochondria membrane potential change by Flowcytometry
E. Apoptosis determination in Mitochondria membrane potential change by confocal microscope
F. Apoptosis determination by H&E Staining Method
G. Apoptosis determination by Giemsa Staining Method
H. Apoptosis determination by Hoechst Staining Method
I. Apoptosis determination by Maygruwalds Regent Staining Method
J. Apoptosis determination by Gel Electrophoresis

In vitro Brine shrimp lethality assay (cytotoxicity assay)

A. By 3 X magnifying glass method
B. By 24 well method

In vitro Antioxidant activities

A. Antioxidant activity By DPPH (2,2-diphenyl-l-picrylhydrazyl)
      a) 96 well method
      b) By spectroscopy method
B. Antioxidant activity By Nitric Oxide scavengingactivity
C. Antioxidant activity By ABTS (2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid)
D. Antioxidant activity By Superoxide scavenging activity
E. Antioxidant activity By FRAP Assay
F. Antioxidant activity By Reducing power

In vivo Antioxidant activities Enzymes From Tissue

A. In vivo Antioxidant activities of Superoxide (SOD)
B. In vivo Antioxidant activities of CATA LASE
C. In vivo Antioxidant activities of GSH (Glutathione)
D. In vivo Antioxidant activities of LPO
E. In vivo Antioxidant activities of TP (Total Protein)
F. In vivo Antioxidant activities of Glutathione Peroxidase

Non Enzymatic Antioxidant

A. Estimation of Vitamin C
B. Estimation of SOD
C. Estimation of TBA
D. Estimation of OH radical

In vitro Antidiabetic activities

A. Antidiabetic activity by Alpha amylase inhibitory Assay
B. Antidiabetic activity By Anti-glycation Method
C. Antidiabetic activity By glucose uptake Assay
D. Antidiabetic activity By Glucosidase inhibition Method
E. Antidiabetic activity dialysis method
F. Antidiabetic activity Glucose Binding Assay

In vitro Anti-inflammatory activity

A. Anti-inflammatory activity By protein denaturation Method
B. Anti-inflammatory activity By Membrane Stabilizing Activity Anti-inflammatory activity By heat Induced C. hemolytic Method Anti-inflammatory activity By Bovine Albumin Fraction Method Anti-inflammatory activity D. By Trypsin Inhibitory assay BY BSA Anti-inflammatory activity By Proteinase inhibition By Using Casein
E. Anti-inflammatory activity on cell line

In vitro Anti-Maleria activity

A. Assay of Beta Hematin formation inhibition by UV
B. Assay of Beta Hematin formation inhibition by IR

In vitro Antimicrobial activity

A. Antimicrobial activity by Agar well method
B. Antimicrobial activity by Disc Diffusion
C. Antimicrobial activity by MIC (Minimum Inhibitory Concentration)
D. Antimicrobial activity by Anti-biofilm method by 96 wellplate method
E. Antifungal Activity by Agar well method
F. Antifungal Activity by Disc Diffusion)
G. Biochemical Test for Microorganisms

In vitro anti-obesity activity

A. Lipase inhibition assay
B. In vitro cholesterol Inhibition assay

In vitro Anti-urolithiasis activity

A. COM (Crystal Aggregation assay) (Kidney stone inhibition assay)
B. Nucleation assay

In vitro Genotoxicity

A. Antimitotic activity

In vitro Anti-ulcer Activity

A. H+ K+ ATPase inhibition assay
B. Acid neutralization capacity
C. Urease inhibition assay

In vitro Immuno-Modulatory

A. Phagocytosis assay by using Cabdida albican

In vitro Putative Anti-HIV activity

A. In vitro pepsin inhibition assay

In vitro Anthelmintic activity

A. In vitro Anthelmintic activity by using Pheretima Posthuma
B. In vitro Anthelmintic activity by using Eisenia fetida

In vitro Scratch /Wound-Healing activity

A. In vitro Scratch /Wound-Healing activity by using cell line

TLC

A. Synthetic Steroid from Ayurvedic Formulation
B. Estimation Of Alkaloids
C. Estimation Of Glycosides
D. Estimation Of Flavonoids
E. Estimation Of Tannins
F. Estimation Of Sapiens
G. Estimation Of Carotenoid

Pharmacognostic evaluation

A. TS/LS
B. Powder microscopy

Extraction

A. Aqueous extraction by maceration (100 gm)
B. Soxhlet extraction (lOOgm)
C. Preparation of ARK / Asava/ Arishata (100 gm)
D. Oil separation (Essential/ Volatile) by using Clevenger’s apparatus

Phytochemical screening (Minimum 10 test)

     a)Quantification
A. Total phenolic content
B. Total tannin Content
C. Total Saponin content
D. Total flavonoid Content

Standardization of drugs

A. Organoleptic properties (Colour, odor, taste, appearance)
B. Physico chemical Properties (pH, LOD, foreign matter, Saponification value, Acid insoluble ash, alcohol soluble extractives and water soluble extractives and volatile oil)
C. Physico chemical Properties oil or liquid (pH, foreign matter, Saponification value, Viscosity, Refractive index, Saponification value, iodine value, alcohol soluble extractives values and water soluble extractives values)

Stability evaluation by Microbiological aspects

A. Smear Examination
B. Culture study *(A- Fungal Culture , B- Aerobic culture)

Plasma /Serum Estimation Parameter

A. IL-6 estimation Kit
B. TNF-alpha estimation Kit
C. COX-I and II Inhibition Assay
D. Rat Adeponectin estimation Kit
E. Human Collagen Type IV estimation Kit
F. Rat Resistin estimation Kit
G. Rat Leptin estimation Kit
H. Rat Erythropoietin estimation Kit
I. Rat transferrin estimation Kit
J. Lipid Peroxidation Inhibition Assay byTBARS
K. GPT Estimation
L. LFT (SGOT, SGPT, ALP, ACT)
M. KFT (Creatinine, Bilirubun, Urea)
N. Lipid profile (Cholesterol, HDL, LDL, VLDL, Triglycerides)

In vitro Alzheimer activity

A. In vitro Alzheimer activity by using Anticholinesterase enzyme
B. In vitro Alzheimer activity by using Cell line

HET CAM assay

A. In vitro angiogenesis inhibition assay

Anti-cancer activity

A. Tryphan blue dye exclusion method
B. Cell Viability Assay by MTT
C. Cell Viability Assay by SRB
D. Cell Viability Assay by XTT
E. Cell Viability Assay by WST
F. Cytotoxic Evolution In Neutrophils By Cell Morphology
G. Cell Viability Assay by Crysta

In vitro Apoptosis Determination

A. Apoptosis determination by AO/EB Staining Method
B. Apoptosis determination by DNA Fragmentation using Flow cytometry (out soure)
C. Apoptosis determination by DAPI Staining
D. Apoptosis determination in Mitochondria membrane potential change by Flowcytometry (out soure)
E. Apoptosis determination in Mitochondria membrane potential change by confocal microscope
F. Apoptosis determination by H&E Staining Method
G. Apoptosis determination by Giemsa Staining Method
H. Apoptosis determination by Hoechst Staining Method
I. Apoptosis determination by Maygruwalds Regent Staining Method
J. Apoptosis determination by Gel Electrophoresis

In vitro Apoptosis Determination

A. Apoptosis determination by AO/EB Staining Method
B. Apoptosis determination by DNA Fragmentation using Flow cytometry (out soure)
C. Apoptosis determination by DAPI Staining
D. Apoptosis determination in Mitochondria membrane potential change by Flowcytometry (out soure)
E. Apoptosis determination in Mitochondria membrane potential change by confocal microscope
F. Apoptosis determination by H&E Staining Method
G. Apoptosis determination by Giemsa Staining Method
H. Apoptosis determination by Hoechst Staining Method
I. Apoptosis determination by Maygruwalds Regent Staining Method
J. Apoptosis determination by Gel Electrophoresis

InVivo Pharmacological Services approved by CCSEA
  1. Development and Screening of Animal model (Anticancer, Antidiabetic, Anti-inflammatory)
  2. Biochemical, Hematological & Histopathological studies (Slide prepatation, Capturing Images & Interpretation)
  3. Streptozotocin-induced type I diabetes in rats
  4. Alloxan-induced type I diabetes in rabbits
  5. Streptozotocin and high fat died-induced type II diabetes in rats
  6. Triton-X induced hyperlipidemia in rats
  7. Complete Freund’s adjuvant-induced arthritis in rats
  8. Monosodium iodoacetate-induced osteoarthritis in rats
  9. Dextran sodium sulfate-induced colitis in mice
  10. TPA-induced psoriasis-like lesions in mice
  11. Imiquimod induced psoriasis in mice
  12. Carrageenan induced paw edema in rats
  13. Paclitaxel-induced neuropathic pain in mice
  14. Formalin induced writhing in mice
  15. Ethylene glycol-induced nephrolithiasis in rats
  16. Pylorus ligation-induced gastric ulceration in rats
  17. Carbon tetrachloride induced hepatotoxicity in rats
  18. Thioacetamide-induced hepatotoxicity in rats
  19. Aminotriazole-induced hypothyroidism in rats
  20. Scopolamine-induced memory impairment in mice
  21. Chronic unpredictable mild stress-induced depression in rats
  22. Cyclophosphamide induced thrombocytopenia in mice
  23. Phenylhydrazine induced hemolytic anemia in rats
  24. Acute and Subacute toxicity studies in rats in accordance with OECD guidelines
Analytical Services (Outsourcing)
  1. UV Analysis, IR, NMR, GCMS
  2. LCMS/ HRMS Analysis
  3. Single Crystal X-ray
  4. Particle Size & Zeta Potential Analysis
  5. HPLC Analysis
  6. HPTLC Analysis
Ayurvedic Formulation analysis
  1. Phyto-chemical extraction
  2. Phyto-chemical analysis
  3. TLC finger printing
  4. Isolation & characterization of Phyto constituents
  5. Standardization of Ayurvedic drugs (As per API)
Hands on training
  1. Biotechnology Section: Demonstration on Tissue & Cell culture, Gel electrophoresis
  2. Microbiology Section: Novel technique in microbiology study like MIC, MBC, Antibiofilm etc
  3. Analytical instruments handling
  4. Animal Handling and dosing administration

We provide Project Assistance to students of B.Pharm., M.Pharm., B.Sc., M.Sc., M.B.B.S., B.A.M.S., B.D.S. and Ph.D. programme